Composite
Part:BBa_K1315009:Design
Designed by: Dimitrios Michailidis Group: iGEM14_Dundee (2014-09-26)
Pseudomonas AutoInducer 1 (PAI-1) Biosensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 849
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 382
Illegal AgeI site found at 579 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1598
Design Notes
All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
Source
All the parts have come from existing iGEM distribution kits.